Composite
Part:BBa_K3888999:Design
Designed by: Xiaoxuan Cheng Group: iGEM21_SJTang (2021-10-20)
HupS knockout
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 1521
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 1521
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 1521
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 1521
Illegal NgoMIV site found at 121
Illegal NgoMIV site found at 258
Illegal NgoMIV site found at 690
Illegal NgoMIV site found at 1068 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 414
Design Notes
Our flanking region is about 1kbp in length for deletion.
Source
the genome of R. Palustris