Coding

Part:BBa_K3724009

Designed by: Tracey Moyston   Group: iGEM21_Rochester   (2021-09-30)
Revision as of 01:52, 1 October 2021 by Registry (Talk | contribs)

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Tetraheme cytochrome c protein/ quinol dehydrogenase cymA

Two extracellular electron transfer pathways have been identified in the reduction of graphene oxide (GO) by Shewanella oneidensis. These are indirect electron transfer, mediated by secreted electron shuttles, and direct extracellular electron transfer (DET) which involves direct contact with the extracellular material. The DET pathway involves c-type cytochromes in the bacterial membrane which includes cymA. cymA is an inner membrane protein that is essential for the reduction of extracellular materials. It accepts electrons from the cytoplasmic menaquinone pool where the electrons are then shuttled to outer membrane proteins for extracellular reduction.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 10
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 7
    Illegal BsaI.rc site found at 583


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