Composite

Part:BBa_K3733046

Designed by: Zhenhao Han   Group: iGEM21_HZAU-China   (2021-10-01)
Revision as of 20:50, 1 October 2021 by Registry (Talk | contribs)

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RNA Thermometer(heat-inducible) test system

Use a constitutive promoter (BBa_J23110), RNA thermometer (BBa_K3247005), strong RBS (BBa_B0034), fluorescent protein neGFP (BBa_K3733012),and an artificial terminator (BBa_B1006). Will express high levels of fluorescent protein above 37 ℃. Source:Used a strong RBS to produce high levels of expression of the neGFP fluorescent protein.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 158
    Illegal AgeI site found at 281
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None