Composite

Part:BBa_K3981014

Designed by: Yulin Peng   Group: iGEM21_AHUT_China   (2021-10-14)
Revision as of 12:10, 14 October 2021 by Registry (Talk | contribs)

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pET28a-his-linker-ISZ-sTRAIL

This plasmid is our improved part based on the new part. This improved part expresses sTRAIL with a trimerization domain, which can better allow sTRAIL to function. We constructed this plasmid for the purpose of expressing sTRAIL alone. To make a comparison, see which protein expressed has the better effect on cancer cells and the more active one. And compare with the plasmid with Her2 to reflect the targeting effect of Her2 on cancer cells.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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