Composite

Part:BBa_K3738025:Design

Designed by: Mark Lea   Group: iGEM21_Lethbridge   (2021-10-20)
Revision as of 17:16, 20 October 2021 by Registry (Talk | contribs)

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McyH complementary Lbu-crRNA (Composite)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This composite part includes the IPTG-inducible T7 promoter (BBa_J64997), McyH Complementary Lbu-crRNA (BBa_K3738023), and double terminator BBa_B0015.

In design of crRNA, ensure the final complementary base is not a G.

The part must be in vitro transcribed to obtain the direct-repeat stem loop RNA structure and complex with Cas13a.


Source

The Mcy gene cluster comes from Microcystic Aeruginosa, however the crRNA originates from Leptrotrichia buccalis

References