Composite

Part:BBa_K3739085

Designed by: Shichen Geng   Group: iGEM21_XMU-China   (2021-09-30)
Revision as of 19:00, 30 September 2021 by Registry (Talk | contribs)

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K525998(T7-RBS)-LMT-his-hutH-FT

Histidine ammonia-lyase with a his-tag is involved in catalyzing the reaction of changing L-histidine into trans-urocanate. LMT is a signal peptile. Direct fluorescent labeling of tetracysteine-motif-tagged secretory proteins with the biarsenical compound FlAsH is possible in situ without the need to recover the cell-free supernatant.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 257
    Illegal NgoMIV site found at 693
    Illegal NgoMIV site found at 1428
    Illegal NgoMIV site found at 1664
    Illegal AgeI site found at 330
    Illegal AgeI site found at 1157
    Illegal AgeI site found at 1653
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None