Part:BBa_K3725220
Phosphorylated NarL-Specific w/ GFP Reporter
Lambert_GA iGEM 2021’s nitrate biosensor is modeled after E.coli’s natural nitrate sensor, the Nar Operon, which includes the membrane-bound sensor protein NarX and its corresponding DNA-binding response regulator NarL. The NarL/NarX system detects nitrate by using phosphorylated NarL as an inducer of promoter part BBa_K3725210 (Promoter pNar). In the presence of nitrate, NarX phosphorylates NarL, which activates pNar, and sfGFP is transcribed. Conversely, in the absence of nitrate, NarX does not phosphorylate NarL and sfGFP is not transcribed. To analyze the concentration of nitrate present in a solution, nitrate levels need to be correlated with a reporter protein, or a promoter responsive to phosphorylated NarL and report downstream GFP, a well-characterized fluorescent protein.
Usage and Biology
Lambert_GA iGEM 2021’s nitrate biosensor is modeled after E.coli’s natural nitrate sensor, the Nar Operon, which includes the membrane-bound sensor protein NarX and its corresponding DNA-binding response regulator NarL. The NarL/NarX system detects nitrate by using phosphorylated NarL as an inducer of promoter part BBa_K3725210 (Promoter pNar). In the presence of nitrate, NarX phosphorylates NarL, which activates pNar, and sfGFP is transcribed. Conversely, in the absence of nitrate, NarX does not phosphorylate NarL and sfGFP is not transcribed. To analyze the concentration of nitrate present in a solution, nitrate levels need to be correlated with a reporter protein, or a promoter responsive to phosphorylated NarL and report downstream GFP, a well-characterized fluorescent protein.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 930
Illegal BsaI site found at 1108
Illegal SapI.rc site found at 133
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