Composite
Part:BBa_K3733044
Designed by: Zhenhao Han Group: iGEM21_HZAU-China (2021-10-01)
Toxin/antitoxin HepT/MntA suicide system working at low temperatures
Use a constitutive promoter (BBa_J23110), RNA thermometer (BBa_K3247005), strong RBS (BBa_B0034), antitoxin MntA (BBa_K3733009), toxin HepT (BBa_K3733010),and a strong transcriptional terminator Lambda t1 transcriptional terminator (BBa_K864601). Will express the HepT toxin below 28 ℃ to commit suicide and will not commit suicide above 37 ℃ because of the expression of MntA.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//awards/composite_part/nominee
None |