Regulatory
pNar

Part:BBa_K3725210

Designed by: Adam Silverman   Group: iGEM21_Lambert_GA   (2021-09-30)
Revision as of 16:52, 20 October 2021 by Saifkhan (Talk | contribs)


Phosphorylated NarL-Specific Promoter Sequence (pNar)

pNar is a regulatory promoter responsive to phosphorylated NarL. This gene is sourced from Bacillus Subtilis; however, it works with the DNA-binding response regulator NarL from the E. coli Nar Operon.


Usage and Biology

Lambert_GA iGEM 2021 developed a cell-free nitrate biosensor, using the pNAR promoter to quantify the amount of nitrate present in a solution. Lambert_GA’s part BBa_K3725220is a composite part with sfGFP driven by this pNar promoter. The team’s biosensor utilized three plasmids, NarL, NarX and a sfGFP part with the pNAR promoter. This was designed by Dr. Adam Silverman at the Jewett Lab at Northwestern University and shipped to our lab for testing.

==Sequence and Features==


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

This sequence is only the promoter sequence. For the composite part with sfGFP, visit part BBa_K3725220.


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Categories
Parameters
None