Coding

Part:BBa_K3712000

Designed by: Yifan Li   Group: iGEM21_HUST2-China   (2021-10-19)
Revision as of 00:42, 20 October 2021 by Registry (Talk | contribs)

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a kind of elastin like peptide (VPGIG)27

It is a kind of elastin like peptide we designed. N means the number of VPGIG repeats. ELP (Elastin-like polypeptide) is a thermally responsive polymer that changes phase when it reaches a critical temperature. When the temperature is higher than the critical temperature, ELP will aggregate through hydrophobic action, and the hydrophobic tail will be surrounded in the interior while the hydrophilic head will be exposed. But ELP remains soluble below the critical temperature. ELP is composed of pentapeptide repeat sequence Val-Pro-Gly-Xaa-Gly (Xaa is any amino acid except Pro)[1], and its transition temperature can be controlled by the concentration, the repeat number of ELP repeat sequence and the amino acid type at Xaa. Through theoretical prediction[2], we predict 10mg/L VPGIG27 aggregates at 42℃, which is the temperature used in acne heat treatment, so that heat treatment can combine with our drugs.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None