Coding

Part:BBa_K3863007

Designed by: LEONG WENG I   Group: iGEM21_PuiChing_Macau   (2021-09-26)
Revision as of 14:39, 26 September 2021 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


PHA-flag-PCT-HA

1. PHA Synthase (BBa_K1211002 from iGEM 2013 iGEM13_Yale) 

  1. Function: This is the coding sequence for a Polyhydroxyalkanoate synthase from Pseudomonas resinovorans. The enzyme takes the monomer (D)-lactyl-CoA and creates the PLA homopolymer. This sequence includes four mutations which were found to increase yields of PLA (Yang et al.)

  2. Full name: Pseudomonas resinovorans Polyhydroxyalkanoate synthase

2. PCTcp (BBa_K1211001 from iGEM 2016 iGEM16_Evry) 

  1. Function: This is the coding sequence for class II poly(R)-hydroxyalkanoic acid (PHA) synthase 1 from Pseudomonas resinovorans. The enzyme takes the monomer (D)-lactyl-CoA and produce the PLA homopolymer.This sequence is codon optimized for Pseudomonas putida and includes four amino acid mutations (E130D/S325T/S477G/Q481K) which were found to increase yields of PLA [1,2]

  2. Full name: Pseudomonas resinovorans PHA synthase 1

  lac operator   RBS  PHA Synthase (BBa_K1211002 )   Flag tag  T7 promotor  lac operator  RBS  PCTcp  HA tag  T7 terminator f1 ori Ampr      Ampr promotor   ori rop    lacI     lacI promotor   T7 promotor

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1411
    Illegal PstI site found at 25
    Illegal PstI site found at 118
    Illegal PstI site found at 208
    Illegal PstI site found at 280
    Illegal PstI site found at 742
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1411
    Illegal PstI site found at 25
    Illegal PstI site found at 118
    Illegal PstI site found at 208
    Illegal PstI site found at 280
    Illegal PstI site found at 742
    Illegal NotI site found at 1708
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1411
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1411
    Illegal PstI site found at 25
    Illegal PstI site found at 118
    Illegal PstI site found at 208
    Illegal PstI site found at 280
    Illegal PstI site found at 742
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1411
    Illegal PstI site found at 25
    Illegal PstI site found at 118
    Illegal PstI site found at 208
    Illegal PstI site found at 280
    Illegal PstI site found at 742
    Illegal NgoMIV site found at 1167
    Illegal NgoMIV site found at 1212
    Illegal AgeI site found at 448
    Illegal AgeI site found at 853
    Illegal AgeI site found at 1486
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 251


[edit]
Categories
Parameters
None