Regulatory
pNar

Part:BBa_K3725210

Designed by: Adam Silverman   Group: iGEM21_Lambert_GA   (2021-09-30)
Revision as of 16:27, 20 October 2021 by Saifkhan (Talk | contribs)


Phosphorylated NarL-Specific Promoter Sequence (pNar)

pNar is a regulatory promoter responsive to phosphorylated NarL. This gene is modeled after the E.coli natural nitrate sensor, the Nar Operon, which includes the membrane-bound sensor protein NarX and its corresponding DNA-binding response regulator NarL.


Usage and Biology Lambert_GA iGEM 2021 developed a cell-free nitrate biosensor, using the pNAR promoter to quantify the amount of nitrate present in a solution. Lambert_GA’s part BBa_K3725220 is a composite part with sfGFP driven by this pNar promoter. The team’s biosensor utilized three plasmids, NarL, NarX and a sfGFP part with the pNAR promoter. These plasmids were designed by Dr. Adam Silverman at the Jewett Lab at Northwestern University and shipped to our lab for testing.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

This sequence is only the promoter sequence. For the composite part with sfGFP, visit part BBa_K3725220.


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Categories
Parameters
None