Composite

Part:BBa_K3733043

Designed by: Zhenhao Han   Group: iGEM21_HZAU-China   (2021-10-01)
Revision as of 20:44, 1 October 2021 by Registry (Talk | contribs)

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HepT suicide system working at low temperatures

Use a constitutive promoter (BBa_J23110), RNA thermometer (BBa_K3733011), strong RBS (BBa_B0034), toxin HepT (BBa_K3733010), and a strong transcriptional terminator Lambda t1 transcriptional terminator (BBa_K864601). Will express the HepT toxin below 28 ℃ to commit suicide.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 82


[edit]
Categories
Parameters
None