RNA

Part:BBa_K3815011

Designed by: Hiroto Koga   Group: iGEM21_Kyoto   (2021-10-18)
Revision as of 15:32, 20 October 2021 by Koga (Talk | contribs)


Part of the V-ATPaseB gene of Frankliniella occidentalis to synthesize dsRNA for RNAi This is the Part of the V-ATPaseB gene of Frankliniella occidentalis to synthesize dsRNA for RNAi. To product dsRNA this sequence inserted in L4440 plasmid, and transformed into HT115(DE3).



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 452
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 320


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Parameters
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