Protein_Domain

Part:BBa_K3896010

Designed by: Zihan Wu   Group: iGEM21_NEU_CHINA   (2021-10-01)
Revision as of 08:30, 9 October 2021 by Tjm (Talk | contribs)


HCoV-229E RBD with His-tag

The target DNA can express a 121 amino acid protein, which is a segment of 229E-CoV RBD, including Lys201 to Ser321, and is a key part of binding to the receptor hAPN. The C-terminal has his tag, which is convenient for subsequent protein extraction and purification. The purified protein can specifically bind to pmrCAB (hAPN) to trigger downstream reaction.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 391
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 211
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None