Coding

Part:BBa_K3739082

Designed by: Shichen Geng   Group: iGEM21_XMU-China   (2021-09-30)
Revision as of 18:38, 30 September 2021 by Registry (Talk | contribs)

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Aly01-his-hutH-FT

Histidine ammonia-lyase with a his-tag is involved in catalyzing the reaction of changing L-histidine into trans-urocanate. Aly01 is a signal peptile. Direct fluorescent labeling of tetracysteine-motif-tagged secretory proteins with the biarsenical compound FlAsH is possible in situ without the need to recover the cell-free supernatant.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 237
    Illegal NgoMIV site found at 673
    Illegal NgoMIV site found at 1408
    Illegal NgoMIV site found at 1644
    Illegal AgeI site found at 73
    Illegal AgeI site found at 310
    Illegal AgeI site found at 1137
    Illegal AgeI site found at 1633
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None