Coding

Part:BBa_K3815000

Designed by: Hiroto Koga   Group: iGEM21_Kyoto   (2021-10-19)
Revision as of 15:18, 20 October 2021 by Shiotsu (Talk | contribs)


Mxe GryA intein-PT-linker-ELK16 for peptide production

This part is for new purification method, ELK16 system. This part is composed of ELK16, Mxe Gyr intein, and PT linker. When this fused protein is produced, ELK16 self-assembles and precipitates. After that, taking this aggregate and adding DTT to it, the N terminal of Mxe GyrA intein is cut. Then, we can get the targeted protein.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 13
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 481
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None