Part:BBa_K3885171

Interacting Guide RNA（igRNA）

The rational design of igRNA is adding additional sequences in the 5' of sgRNA.

Usage and Biology

Figure 1. Schematic of gRNA and igRNA.

The “igRNA” stands for RNA-interacting guide RNA, which switch to their active state only upon interaction with target RNAs (Figure 1).
The additional sequences include recognition sequence A, cis-repressed sequence and recognition sequence B. The cis-repressed sequence is complementary to the spacer, leading to the repression of the binding ability between igRNA with its DNA target. The recognition sequence A and the recognition sequence B are complementary to the interacting sequence A and interacting sequence B, respectively.

All designed igRNAs were analyzed using NUPACK webserver.
The igRNA that has lower free energy, co-folding energy with target RNA as well as reduced self-comlementarity at 29℃ would be the best (Figure 2).

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Unknown
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]