Coding

Part:BBa_K4035002

Designed by: Anissa Hammi   Group: iGEM21_EPFL   (2021-08-31)
Revision as of 05:41, 13 September 2021 by Hammi (Talk | contribs) (→‎Usage and Biology)


Dimerization of the copper metallothionein 1 : CUP1-(GGGGS)3-CUP1

This protein is made of two yeast copper metallotionein protein, CUP1 ((BBa_M45090), linked together by a flexible linker made of three times the GGGGS amino acid sequence.

Usage and Biology

Copper metallotionein CUP1 (BBa_M45090) is a protein responsible for copper binding protein in the yeast Saccharomyces cerevisiae. In order to increase the copper retrieval efficiency, two copies of CUP1 were linked together and expressed at the outter surface of S. cerevisiae (BBa_K4035009). The GGGGS is a common flexible linker and was inserted 3 times in order to allow distances between the two copies of CUP1 and avoid interaction.

Characterization

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 190
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//binding/metal
//cds/membrane/extracellular
//chassis/eukaryote/yeast
Parameters
biologyCUP1
chassisS.cerevisiae
controlpCTcon2V5, WT EBY100
directionForward
functioncopper binding protein