Tag

Part:BBa_K3815015

Designed by: Alexander Liu   Group: iGEM21_Kyoto   (2021-10-20)
Revision as of 12:13, 20 October 2021 by AlexanderLiu (Talk | contribs)


AANDENYALGA. mutant SsrA degradation tag

This is an engineered derivative of wildtype ssrA tag from Escherichia coli, acquired by mutagenizing the WT tag with random-base primers. Compared to the WT, three C-terminal amino acids LAA were replaced to LGA.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None