Coding

Part:BBa_K3815009

Designed by: Hiroto Koga   Group: iGEM21_Kyoto   (2021-10-16)
Revision as of 09:37, 17 October 2021 by Shiotsu (Talk | contribs)


NOP1-Mxe GryA intein-PT-linker-His tag

This part is made of Hist tag for the peptide production. After producing peptide with His tag, it is recovered by Ni chromatography. Finally, adding DTT to it, we cut the N terminal of Mxe GyrA intein. Then, we can get the targeted protein.In this part, the purpose protein is NOP1.This is a small peptide that can prevent the plants from accepting ethylene. In our experiment, we used it to inhibit the acceptance of ethylene and aging of cut flowers.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 117
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 117
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 117
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 117
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 117
    Illegal NgoMIV site found at 550
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None