Coding

Part:BBa_K3815008

Designed by: Hiroto Koga   Group: iGEM21_Kyoto   (2021-10-16)
Revision as of 09:35, 17 October 2021 by Shiotsu (Talk | contribs)


LL37-Mxe GryA intein-PT-linker-His tag

This part is made of Hist tag for the peptide production. After producing peptide with His tag, it is recovered by Ni chromatography. Finally, adding DTT to it, we cut the N terminal of Mxe GyrA intein. Then, we can get the targeted protein.In this part, the purpose protein is LL37 derived from human. This is antimicrobial peptide that can inhibit the growth of both gram-negative bacteria and gram-positive bacteria. In our experiment, we used it to kill the bacteria in the vase water.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 637
    Illegal AgeI site found at 130
    Illegal AgeI site found at 331
    Illegal AgeI site found at 765
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None