Part:BBa_K1373000

This part encodes NAD synthetase

Open reading frame of nadE

Intracellular redox state of electricity active cells (EAC) is one of the most important physiological traits of extracellular electron transfer efficiency. In particular, the NAD⁺(H) pool size plays a central role of most metabolic pathways. By overexpressing the NAD synthetase, encoded be gene nadE and catalyzes the final step in de novo synthesis and salvage pathway of NAD biosynthesis (Fig. 1), the NAD+ level is increased thereby up-regulating genes whose products catalyze NADH synthesis. Therefore the augmented pool size of NAD⁺(H) result in promotion of NADH(the carrier of electrons)level, leading to high generation of intracellular releasable electrons and better electricity performance of EAC. ^[1]

Fig. 1 De novo synthesis and salvage pathway of NAD biosynthesis.

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