Coding
Part:BBa_K3765001:Design
Designed by: Dipayan Akhuli Group: iGEM21_IISc-Bangalore (2021-10-14)
Revision as of 10:27, 20 October 2021 by Dipayan akhuli (Talk | contribs)
SpyCatcher002
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 150
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 235
Design Notes
Codon optimized for E. coli.
Source
Modified protein fragment. Engineered from the second immunoglobulin-like collagen adhesin domain (CnaB2) of the fibronectin binding protein FbaB from Streptococcus pyogenes.