Part:BBa_K3815005
NOP1v-Mxe GryA intein-PT-linker-ELK16
Descriotion of this part
Targeted protein
This part is for the purification of NOP1v. This is a small peptide that can prevent the plants from sensing ethylene by binding its receptor. In our experiment, we used it to inhibit the acceptance of ethylene that promotes the wilting of a plant and aging of cut flowers. We could not get NOP1 sufficiently when using BBa_K3815033, so we added a valine to the N-terminus of NOP1 according to N-end-rule.
Sequence and Features
Purification system
In order to purify the peptide, we adopted ELK16 system that the past iGEM teams had not used. This part is composed of ELK16, Mxe Gyr intein, and PT linker. When this fused protein is produced, ELK16 self-assembles and precipitates. After that, taking this aggregate and adding DTT to it, the N terminal of Mxe GyrA intein is cut. Then, we can get the targeted protein.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 120
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 120
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 120
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 120
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 120
Illegal NgoMIV site found at 553 - 1000COMPATIBLE WITH RFC[1000]
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