Coding

Part:BBa_K3815005

Designed by: Shiotsu Koudai   Group: iGEM21_Kyoto   (2021-10-16)
Revision as of 09:26, 17 October 2021 by Shiotsu (Talk | contribs)


NOP1v-Mxe GryA intein-PT-linker-ELK16

This part is used for ELK16 system to purify the peptides. When this fused protein is produced, the part of the ELK16 self-assembles and precipitates. After that, taking this aggregate and adding DTT to it, we cut the N terminal of Mxe GyrA intein. Then, we can get the targeted protein. In this part, the targeted protein is NOP1v.This is a small peptide that can prevent the plants from accepting ethylene. In our experiment, we used it to inhibit the acceptance of ethylene and aging of cut flowers. We could not get NOP1 sufficiently when using BBa_K3815033, so we added a valine to the N-terminus of NOP1 according to N-end-rule.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 120
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 120
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 120
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 120
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 120
    Illegal NgoMIV site found at 553
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None