Coding
Part:BBa_K3815004
Designed by: Shiotsu Koudai Group: iGEM21_Kyoto (2021-10-15)
NOP1-Mxe GryA intein-PT-linker-ELK16
Descriotion of this part
Targeted protein
This part is for the purfication of the small peptide, NOP1. It can prevent the plants from accepting ethylene. In our experiment, we used it to inhibit the acceptance of ethylene that promotes the wilting of a plant and aging of cut flowers.
Purification system
In order to purify the peptide, we adopted ELK16 system that the past iGEM teams had not used. This part is composed of ELK16, Mxe Gyr intein, and PT linker. When this fused protein is produced, ELK16 self-assembles and precipitates. After that, taking this aggregate and adding DTT to it, the N terminal of Mxe GyrA intein is cut. Then, we can get the targeted protein.
Usage and Biology
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 117
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 117
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 117
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 117
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 117
Illegal NgoMIV site found at 550 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |