Coding
alpha-gal

Part:BBa_K4032000

Designed by: Tsubasa Okada   Group: iGEM21_Gunma   (2021-09-30)
Revision as of 08:38, 20 October 2021 by Tsugumi (Talk | contribs)


α-galactosidase


This part contains E.coli alpha-galactosidase gene melA (see info https://www.ncbi.nlm.nih.gov/gene/948636)


Usage and Biology

This enzyme catalyzes the hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids.

https://www.uniprot.org/uniprot/P06720


The lac promoter, the RBS and the double terminator are added to this part, forming BBa_K4032xx.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None