Part:BBa_K3735013
Coding sequence
In order to improve the quality of SeNPs and improve the reduction efficiency, we hope to link those standard parts and then, two genes express at the same time. This part consist of low intensity SefA and low intensity ssuE. After verifying the function of SefA and ssuE separately, we integrate them and obtain nine combinations of different promoter strengths. And the low SefA and the low ssuE Can produce the best SeNPs.
In order to explore the optimal conditions for the production of SeNPs,we used the Response surface model.We hope to maximize SeNPs production by optimizing temperature, pH and selenite concentration. First, we conducted a preliminary experiment on these three factors, and we chose the best three gradients(Fig.1) of these three factors to do further experiments.
Fig.1 Preliminary experiment results of response surface experiment. A: pH; B: concentration of selenite; C: Temperature. The results of preliminary experiment show that the SeNPs yield is the best when pH are 6,7,8 temperature are 16℃,30℃,37℃ and selenite concentration are 12mM,15mM,18mM.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
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Illegal NheI site found at 3122 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1537
Illegal BglII site found at 2854
Illegal BglII site found at 3288 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 329
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Illegal NgoMIV site found at 2675
Illegal NgoMIV site found at 2924
Illegal AgeI site found at 671
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Illegal AgeI site found at 2465 - 1000COMPATIBLE WITH RFC[1000]
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