Composite

Part:BBa_K3733027

Designed by: Yuanhui Sun   Group: iGEM21_HZAU-China   (2021-10-01)
Revision as of 14:45, 1 October 2021 by Registry (Talk | contribs)

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The nitrate sensor(J23115-J61100) system with EBS-PyeaR and neGFP

The NarXL is a the two-component signal system in E.coli, which each constitute a sensor histidine kinase and a response regulator. The NarX is sensor histidine kinase, which can phosphorylate NarL in response to nitrate and nitrite. And then the phosphorylated NarL will binding the site and activate the transcription of PyeaR promoter. So in this nitrate sensor system, we use J23115 (weak promoter) and J61100 (weak RBS) to produce NarX and NarL. When we add different concentrations of nitrate, and measurement the fluorescence intensity to reflect the expression level of PyeaR promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 337
    Illegal BglII site found at 505
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1089
    Illegal AgeI site found at 1212
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None