Translational_Unit
Part:BBa_K4002007
Designed by: Nixue Song Group: iGEM21_Xiamen_City (2021-10-12)
Cas9+gRNA+HR-L-endo-pgaA-HR-R
Cas9+gRNA+HR-L-endo-pgaA-HR-R
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 4971
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 688
Illegal BglII site found at 1625
Illegal BglII site found at 6156
Illegal BglII site found at 6714
Illegal BamHI site found at 2443
Illegal XhoI site found at 3981
Illegal XhoI site found at 4584
Illegal XhoI site found at 6674 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 3177
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 6413
Illegal BsaI.rc site found at 5915
Illegal BsaI.rc site found at 6785
[edit]
Categories
Parameters
None |