Protein_Domain
TEVsite

Part:BBa_J18918

Designed by: Raik Gruenberg   Group: Affiliates   (2010-01-26)
Revision as of 17:13, 26 January 2010 by Registry (Talk | contribs)

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TEV cleavage site (E. coli)

TEV cleaves the following AA sequence with high specificity: 

  * Glu-Asn-Leu-Tyr-Phe-Gln↓Gly

but: 

  * Glu-Asn-Leu-Tyr-Phe-Gln↓Ser

is also reported

Note, the part suggested by the Voigt lab, also contains 2 additional 1xGly flanks for increased accessibility.

See also: 

  * BBa_I712077 (TEV N-term)



  * BBa_I712078 (TEV C-term)

  * BBa_I712016 (myri+TEV site, Slovenia team igem2007)

  * BBa_J64007 (Dan Widmaier, Voigt lab)

References

=

Dougherty et al. (1989): Molecular genetic analysis of a plant virus polyprotein cleavage site: a model. PMID: 2669323



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//proteindomain/cleavage
Parameters
None