Composite
Part:BBa_K3771038
Designed by: KAO, YI-CHING Group: iGEM21_NCKU_Tainan (2021-10-01)
PpspA-CS
Description
This composite part is a component of the IFN-γ sensing system and was used to express the taurine production enzyme, CS.
Biology
Binding of IFN-γ to the OmpA/OprF chimeric protein induces the response of the phage shock protein (Psp) system, a highly conserved stress response system in enterobacteria. [1] Signal transduction from the outer membrane to the inner membrane activates the pspA promoter, initiating expression of CSAD. CSAD catalyzes the decarboxylation of L-Cysteine sulfinic acid into hypotaurine, which is spontaneously oxidized to taurine [2].
Fig.1 Taurine pathways in E. coli
Usage
We ligased the cs fragment and pspA promoter on the pSU expression vector and transformed it into DH5α to complete construction of the plasmid.
Characterization
Fig. 2. Colony PCR confirmation of the construction
Reference
1. Darwin AJ. The phage-shock-protein response. Molecular Microbiology. 2005;57(3):621-628. doi:10.1111/j.1365-2958.2005.04694.xhttps://pubmed.ncbi.nlm.nih.gov/16045608/
2. Joo Y-C, Ko YJ, You SK, et al. Creating a New Pathway in Corynebacterium glutamicum for the Production of Taurine as a Food Additive. Journal of Agricultural and Food Chemistry. 2018;66(51):13454-13463. doi:10.1021/acs.jafc.8b05093https://pubmed.ncbi.nlm.nih.gov/30516051/
Sequence and Features
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Categories
Parameters
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