Part:BBa_K792002

Secretion tag from yeast α-factor mating pheromone (MFα1)

This part is the secretion signal peptide from the yeast α-mating factor. This signal peptide directs the secretion of the produced protein, and therefore allows the exportation of it. This peptides are cleaved once the protein is in the lumen of the ER.

To use this tag, attached it directly to your proteins CDS.

See also:

A Kozak sequence from α-factor mating pheromone (MFα1) BBa_K792001

This parts was originally built to be used in [http://2012.igem.org/Team:Buenos_Aires iGEM BsAs 2012 project] to implement a cross-feeding regulatory system between two different yeast strains with specific auxotrophy. [http://2012.igem.org/Team:Buenos_Aires Visit our wiki] to read details about the designing process and implementation details of our system. Also feel free to contact us for any question regarding this part.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Part Contributions

NUS Singapore 2021

Authors:

Tania Santosh Nair, Chew Chin Wei, Linus Tan

Contribution Summary:

Team NUS_Singapore 2021 uccessfully expressed GFP using this part to quantify the performance. Characterization of part performance was also done using a GFP reporter. Check NUS Singapore 2021 wiki to view more information: https://2021.igem.org/Team:NUS_Singapore/Results

File:T--NUS Singapore--results tab1 2.png

Figure 1: Image of the media supernatant, resuspended cells and blank control media under gel visualizer blue light, media and supernatant are clearly fluorescent compared to the control media.

File:T--NUS Singapore--results tab1 1.png

Figure 2: mFa-GFP Assembly schematic of the construct used to characterize performance of this secretion tag.

File:T--NUS Singapore--results tab1 3.png

Figure 3: GFP absorbance of the supernatant and cells of both induced and uninduced pGmFaGFP-H (BY4741), after normalization to blank media. Induced cells secreted approximately 42% of the GFP, while uninduced cells produced and secreted significantly less GFP than induced cells.

Additional Information

Please visit our results page for more information on the characterization data of this part here: https://2021.igem.org/Team:NUS_Singapore/Results