Composite
Part:BBa_K3771022
Designed by: KAO, YI-CHING Group: iGEM21_NCKU_Tainan (2021-10-01)
PpspA-CoaBC
Description
This composite part is a component of the IFN-γ sensing system and was used to express the taurine production enzyme, CoaBC.
Biology
Binding of IFN-γ to the OmpA/OprF chimeric protein induces the response of the phage shock protein (Psp) system, a highly conserved stress response system in enterobacteria. [1] Signal transduction from the outer membrane to the inner membrane activates the pspA promoter, initiating expression of CoaBC. CoaBC converts L-cystate into taurine in the taurine synthesis L-cystate pathway.
Fig.1 Taurine pathways in E. coli
Usage
We ligased the coaBC fragment and pspA promoter on the pSU expression vector and transformed it into DH5α to complete construction of the plasmid.
Characterization
Fig. 2. Colony PCR confirmation of the construction
Reference
1. Darwin AJ. The phage-shock-protein response. Molecular Microbiology. 2005;57(3):621-628. doi:10.1111/j.1365-2958.2005.04694.xhttps://pubmed.ncbi.nlm.nih.gov/16045608/
Sequence and Features
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Categories
Parameters
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