Part:BBa_K3771021
PpspA-JJU10-6xHis
Description
This composite part is a component of the IFN-γ sensing system and was used to express the taurine production enzyme, JJU.
Biology
Binding of IFN-γ to the OmpA/OprF chimeric protein induces the response of the phage shock protein (Psp) system, a highly conserved stress response system in enterobacteria. [3] Signal transduction from the outer membrane to the inner membrane activates the pspA promoter, initiating expression of JJU. JJU converts L-cysteine into L-cystate in the taurine synthesis L-cystate pathway.
Usage
We ligased the jju-6xHis fragment and pspA promoter on the pSU expression vector and transformed it into DH5α to complete construction of the plasmid. The 6xhis allows for confirmation of JJU expression by western blot using the anti-6xHis antibody.
Characterization
圖片描述
Reference
3. Darwin AJ. The phage-shock-protein response. Molecular Microbiology. 2005;57(3):621-628. doi:10.1111/j.1365-2958.2005.04694.x https://pubmed.ncbi.nlm.nih.gov/16045608/
Sequence and Features
| None |