Composite
Part:BBa_M10210
Designed by: Jennifer Brophy Group: Berkeley BioE140L - S09 (2009-05-10)
{Pbad.rbs.prepro.StrepTag}{AG4}{CPG_L6!}{dblTerm}
Composite part with an Arabinose promoter {Pbad}, ribosome binding site {rbs}, prepro periplasmic targetting sequence {prepro}, a streptavidin-binding tag {StrepTag}, a silver binding peptide {AG4}, circularly permutated ompG {CPG_L6} which functions as the carrier protein for display of AG4 on the cell surface of E.coli, and a terminator {dblTerm}
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1249
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1023
Illegal AgeI site found at 2123 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 1005
[edit]
Categories
Parameters
None |