Reporter

Part:BBa_K3889000

Designed by: Prameya Garge, Ashwin Sharma   Group: iGEM21_IISER-Tirupati_India   (2021-09-26)
Revision as of 17:44, 16 October 2021 by AshWinShaRma (Talk | contribs)


Azurite_BFP (BFP.B3)

Azurite is a Blue Fluorescence Reporter protein from Aequorea victoria and has the following parameters [1,2]:

Ex : Peak excitation wavelength (in nm) 383
Em: Peak emission wavelength (in nm) 447
EC: Extinction Coefficient (M-1 cm-1) 26,200
QY: Quantum Yield 0.55
Brightness 14.41
pKa 5.0
Maturation (min) 18.0
Lifetime (ns) 3.65



Usage and Biology

Azurite can be used to report protein production or in systems where fluorescence is required like FRET. In BBa_K3889101 we planned to use Azurite as a reporter for P22 production in Bacillus subtilis 168.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

1. Mena, M. A., Treynor, T. P., Mayo, S. L., & Daugherty, P. S. (2006). Blue fluorescent proteins with enhanced brightness and photostability from a structurally targeted library. Nature biotechnology, 24(12), 1569–1571. https://doi.org/10.1038/nbt1264

2. https://www.fpbase.org/protein/azurite/

[edit]
Categories
Parameters
None