Plasmid_Backbone

Part:BBa_K3846050

Designed by: Marcel Arvid Zimmeck   Group: iGEM21_Hamburg   (2021-10-12)
Revision as of 18:05, 12 October 2021 by Registry (Talk | contribs)

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modified pAM5411 (phytobrick acceptor backbone)

!!! not complete !!!

The broad host range plasmid pAM5411 (RSF1010) was modified to be able to insert phytobricks. Succesful insertion removes eYFP

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 1
    Illegal XbaI site found at 8957
    Illegal PstI site found at 8342
    Illegal PstI site found at 8900
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal NheI site found at 6855
    Illegal PstI site found at 8342
    Illegal PstI site found at 8900
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 1
    Illegal XbaI site found at 8957
    Illegal PstI site found at 8342
    Illegal PstI site found at 8900
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 1
    Illegal XbaI site found at 8957
    Illegal PstI site found at 8342
    Illegal PstI site found at 8900
    Illegal NgoMIV site found at 5909
    Illegal NgoMIV site found at 5919
    Illegal NgoMIV site found at 6898
    Illegal NgoMIV site found at 7040
    Illegal AgeI site found at 4412
    Illegal AgeI site found at 4813
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 8065
    Illegal BsaI.rc site found at 8943


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Parameters
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