Part:BBa_K4061041
Antinsense RNA against Constitutive eGFP
This is an antisense mRNA encoded to downregulate Constitutively expressed (BBa_J23100) eGFP fluorophore. This mRNA will be a part of the composite part (BBa_K4061090). This mRNA was used to check if the mRNA design spanning the protein CDS, RBS and the -10region of promoter was compatible to reduce noise from unwanted fluorophore. With our experiments, we can be sure that this antisense mRNA is very efficient in reduction of noise from Constitutively expressed eGFP. Teams working with BBa_J23100 BBa_B0034 and BBa_K4061002 (Constitutive promoter, Strong RBS and eGFP) could utilise this antisense molecule to suppress noise from the circuit. An additional use is if teams wish to characterise a promoter's activity, they could regulate this antisense mRNA under that promoter and observe the reduction in fluorescence intensity. This can be an indicator of promoter's function.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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