RNA
Part:BBa_K4061095
Designed by: Diya Agrawal Group: iGEM21_HKUST (2021-10-15)
mRFP1 antisense characterisation
This composite part was made to characterise the efficiency of antisense mRNA as designed in BBa_K4061046 in reduction of noise from mRFP1 reporter. We also regulated the antisense mRNA under a PompC promoter (BBa_R0082) to analyse the promoter's function. Rationale being that in the condition when the OmpC promoter should be active (high osmolarity), the antisense mRNA will be more in number, therefore reducing fluorescence intensity. This construct gave us dual information- about antisense mRNA efficiency and the promoter function.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 1085 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1077
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 616
Illegal AgeI site found at 728 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |