Composite
Part:BBa_K3868127
Designed by: Yan Xu Group: iGEM21_NNU-China (2021-10-16)
pYLXP- 18-Nluc
pYLXP- 18-Nluc plasmid contains pYLXP-18, Nluc etc. The recombinant plasmid of pYLXP-18-Nluc was constructed using linearized pYLXP-PTEF-Nluc (digested by AvrII and NheI) and the PCR-amplified promoter pYLXP- 18 sequences by Gibson Assembly.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 294
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 548
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 294
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 294
Illegal NgoMIV site found at 1552 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |