Composite

Part:BBa_K3789010

Designed by: MEI YING YI   Group: iGEM21_UM_Macau   (2021-09-28)
Revision as of 09:01, 28 September 2021 by Registry (Talk | contribs)

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PNP enzyme secretion system

This composite part consists of pGK1 promoter, Mating factor alpha prepro-leader (MF alpha), 6 x glycine, PNP1 coding sequence, and ADH1 terminator. The product of this system is a secretable purine nucleoside phosphorylase(PNP) together with a HA tag. MF alpha enables the enzyme to be secreted. The six glycines between MF alpha and PNP1 coding sequence avoid the interruption between them, enabling them to form correct spatial structure.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1448
    Illegal PstI site found at 1023
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1023
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2273
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1448
    Illegal PstI site found at 1023
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1448
    Illegal PstI site found at 1023
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None