Composite

Part:BBa_K3868108

Designed by: Yan Xu   Group: iGEM21_NNU-China   (2021-10-16)
Revision as of 16:54, 16 October 2021 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


pET-PEW300-eGFP

pET-24a-PEW300 plasmid contains pT7, lacO, PEW300, thrombin, eGFP-His-tag etc.Based on the plasmid of pET-24a, the C-terminal of PEW300 was fused with eGFP in order to characterize the yield of PEW300. Moreover, in order to purify the PEW300, the enzyme loci of thrombin was inserted between PEW300 and eGFP, and the label of 6*His was inserted at the end of eGFP, yielding the plasmid of pET-PEW300-eGFP. The fluorescence intensity of eGFP could be used to indicate the expression level of PEW300.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None