Composite

Part:BBa_K3868106

Designed by: Yan Xu   Group: iGEM21_NNU-China   (2021-10-16)
Revision as of 16:50, 16 October 2021 by Registry (Talk | contribs)

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pET-DCD-1L-eGFP

pET-24a-DCD-1L plasmid contains pT7, lacO, DCD-1L, thrombin, eGFP-His-tag etc.Based on the plasmid of pET-24a, the C-terminal of DCD-1L was fused with eGFP in order to characterize the yield of DCD-1L. Moreover, in order to purify the DCD-1L, the enzyme loci of thrombin was inserted between DCD-1Land eGFP, and the label of 6*His was inserted at the end of eGFP, yielding the plasmid of pET-DCD-1L-eGFP. The fluorescence intensity of eGFP could be used to indicate the expression level of DCD-1L.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 141
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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