Composite

Part:BBa_K3868101

Designed by: Yan Xu   Group: iGEM21_NNU-China   (2021-10-16)
Revision as of 16:37, 16 October 2021 by Registry (Talk | contribs)

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pET-Fa-AMP1-eGFP

pET-24a-Fa-AMP1 plasmid contains pT7, lacO, Fa-AMP1, thrombin, eGFP-His-tag etc.Based on the plasmid of pET-24a, the C-terminal of Fa-AMP1 was fused with eGFP in order to characterize the yield of Fa-AMP1. Moreover, in order to purify the Fa-AMP1, the enzyme loci of thrombin was inserted between Fa-AMP1 and eGFP, and the label of 6*His was inserted at the end of eGFP, yielding the plasmid of pET-Fa-AMP1-eGFP. The fluorescence intensity of eGFP could be used to indicate the expression level of Fa-AMP1.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 111
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 111
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 111
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 111
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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