Plasmid

Part:BBa_K3765011

Designed by: Dipayan Akhuli   Group: iGEM21_IISc-Bangalore   (2021-10-19)
Revision as of 07:31, 19 October 2021 by Registry (Talk | contribs)

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pETMCN-T7

High copy number plasmid; expression under T7 promoter; KanR backbone.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 5105
    Illegal SpeI site found at 5030
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 5111
    Illegal SpeI site found at 5030
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 4964
    Illegal BamHI site found at 5093
    Illegal XhoI site found at 5075
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 5105
    Illegal SpeI site found at 5030
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 5105
    Illegal SpeI site found at 5030
    Illegal NgoMIV site found at 137
    Illegal NgoMIV site found at 3184
    Illegal NgoMIV site found at 3344
    Illegal NgoMIV site found at 4932
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2263


[edit]
Categories
//plasmid
//plasmid/expression/t7
Parameters
None