Composite

Part:BBa_K3868099

Designed by: Yan Xu   Group: iGEM21_NNU-China   (2021-10-16)
Revision as of 16:17, 16 October 2021 by Registry (Talk | contribs)

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pTarget-Donor

pTarget-donnr plasmid contains pj23119, sgRNA-CM etc.The pCas / pTardet-donnr complex can make the related lacI operator gene in DE3 region replaced by the antibiotic gene of chloramphenicol (CM) . Importantly, the gene of CM can also be used as secondary screening pressure to remove false positive strains.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 182
    Illegal XbaI site found at 188
    Illegal SpeI site found at 73
    Illegal PstI site found at 200
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 182
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 50
    Illegal SpeI site found at 73
    Illegal PstI site found at 200
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 182
    Illegal BglII site found at 212
    Illegal XhoI site found at 236
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 182
    Illegal XbaI site found at 188
    Illegal SpeI site found at 73
    Illegal PstI site found at 200
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 182
    Illegal XbaI site found at 188
    Illegal SpeI site found at 73
    Illegal PstI site found at 200
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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