Part:BBa_K3868098

pTargetS

pTargetS plasmid contains pj23119, sgRNA-1 and sgRNA-2 etc.Two different sgRNA expression frames were tandemly linked, allowing GGGGGGGG to be covered, resulting in a more diverse editing outcome.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 182
  Illegal EcoRI site found at 552
  Illegal XbaI site found at 188
  Illegal XbaI site found at 558
  Illegal SpeI site found at 73
  Illegal SpeI site found at 443
  Illegal PstI site found at 200
  Illegal PstI site found at 570
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 182
  Illegal EcoRI site found at 552
  Illegal NheI site found at 7
  Illegal NheI site found at 30
  Illegal NheI site found at 50
  Illegal NheI site found at 377
  Illegal NheI site found at 400
  Illegal NheI site found at 420
  Illegal SpeI site found at 73
  Illegal SpeI site found at 443
  Illegal PstI site found at 200
  Illegal PstI site found at 570
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 182
  Illegal EcoRI site found at 552
  Illegal BglII site found at 212
  Illegal BglII site found at 582
  Illegal XhoI site found at 236
  Illegal XhoI site found at 606
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 182
  Illegal EcoRI site found at 552
  Illegal XbaI site found at 188
  Illegal XbaI site found at 558
  Illegal SpeI site found at 73
  Illegal SpeI site found at 443
  Illegal PstI site found at 200
  Illegal PstI site found at 570
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 182
  Illegal EcoRI site found at 552
  Illegal XbaI site found at 188
  Illegal XbaI site found at 558
  Illegal SpeI site found at 73
  Illegal SpeI site found at 443
  Illegal PstI site found at 200
  Illegal PstI site found at 570
  
• 1000
  COMPATIBLE WITH RFC[1000]