Composite

Part:BBa_K3868097

Designed by: Yan Xu   Group: iGEM21_NNU-China   (2021-10-16)
Revision as of 08:59, 18 October 2021 by Minter (Talk | contribs)


pCBE

The pCBE plasmid contains the lambda operator, cytidine deaminase, Uracil DNA glycosylase inhibitor and LVA degradation labels. The CBE / sgRNA complex can bind to the double-stranded DNA to form an R-loop in a sgRNA and PAM-dependent manner. CDA catalyzes the deamination of cytosines located at the top (non-complementary) strand within 15–20 bases upstream from PAM, which results in C-to-T mutagenesis. Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1572
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1572
    Illegal NheI site found at 1331
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1572
    Illegal BglII site found at 5013
    Illegal BamHI site found at 3610
    Illegal XhoI site found at 4616
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1572
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1572
  • 1000
    COMPATIBLE WITH RFC[1000]


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